The techniques included in the Nanoscopy Platform are excellent tools for the early-stage molecular characterization of biomolecules and ultrastructural analysis of sub-cellular organelles using multi-color fluorescence markers.
Depending on the type of method used, it is possible to improve the maximal resolution of fluorescence-based images from 200 nm to 20-100nm. Hitherto non-resolvable small subcellular structures such as centrioles, lipid rafts, neuronal dendrite spines, nuclear pore complexes, bacterial polar complexes, and many other macromolecular structures with less than 200 nm in Y,X dimension - so far exclusively accessible by electron microscopy - can be visualized by multi color light microscopy, and eventually by live cell imaging.
TIRF illumination for selective visualization of slim structures such as the cell membrane for studies of cell motili...Read article
Flexible system based on standard immunostaining techniques that combine TIRF and epifluorescence with single molecul...Read article